Développement bactérien


INHIBITION OF BACTERIAL DEVELOPMENT

A laboratory specializing in organic farming contacted us following a fertility problem.
In fact, on certain batches, the bacteria placed in culture did not seem to develop even though the culture media were identical.
We studied with him his entire preparation protocol and it turns out that during the cleaning process certain alcohols were used to ensure good disinfection .
However, the alcohols can persist after washing, thus becoming residual solvents which may involve an inhibition of the growth of bacteria .
As you know alcohols are often used to kill bacteria and viruses, which is why they are the main ingredients of hydroalcoholic gels !
To verify this hypothesis, our team carried out a comparative analysis by HS / GCMS with a broad screening of ultra visible compounds, allowing us to highlight the presence or absence of possible residual solvents.
The results of this analysis reveal the presence of various solvents and more particularly that of isopropyl alcohol [67-63-0] in the two batches of flasks, those in conformity and not in conformity.
The difference is that in the non-conforming batch the quantity of isopropyl alcohol [67-63-0] is 10 times greater than that present in the conforming vials.

We therefore hold the culprit of this fertility problem! The isopropyl alcohol [67-63-0] used during the disinfection of the flasks upstream of their use for biological culture has been overdosed or poorly eliminated in the case of non-compliant flasks inducing their sterility.
Our client can now readjust his preparation and washing protocol in order to no longer encounter fertility problems. We support it in the study of the effectiveness of its new cleaning process.
Isopropyl alcohol detection
Are you facing this type of problem, or having compliance issues and suspecting the presence of residual solvents?
Call on the Polymex laboratory!

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