GCMS-FID

GCMS/FID - Perkin Elmer Clarus

Chromatograph : Perkin Elmer Clarus 690
Detector = Mass Spectrometer: SQ8 - Electron Impact Quadrupole (EI).
Column: Elite-5MS (capillary, methyl-silicone grafted with 5% PH ME siloxane), 60 mx 0.25 mm x 0.25 µm & Elite-Wax (capillary, stationary phase in polyethylene glycol), 40 mx 0.18 mm x 0.30 µm .
Injector : Autosampler (x108).
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Chromatograph : Perkin Elmer Clarus 680

Detector 1 = Mass spectrometer: Perkin Elmer Clarus 600T - Electron Impact Quadrupole (EI).

Column : Elite-5MS (capillary, methyl-silicone grafted with 5% PH ME siloxane), 60 mx 0.25 mm x 0.25 µm & Elite-Wax (capillary, stationary phase in polyethylene glycol), 40 mx 0.18 mm x 0.30 µm .
Detector 2 = FID : Autoignition with PPC electronic regulation.

Injector : Autosampler (x84).
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Chromatograph : Perkin Elmer Clarus 580

Mass spectrometer : Perkin Elmer Clarus 560 S - Electron Impact Quadrupole (EI).

Column : Elite-5MS (capillary, methyl-silicone grafted 5% PH ME siloxane), 40 mx 0.18 mm x 0.18 µm.

Application domain

Identification and determination of volatile organic compounds (raw materials, solvents, adjuvants, residual monomers, pollution, etc.).

This device makes it possible to carry out a structural analysis when it is coupled to the pyrojector and an ultra-volatile analysis when it is coupled to a headspace.

Principle

Separation and identification technique reserved for the analysis of relatively volatile and thermally stable, non-ionic compounds with a molar mass of less than 650 g/mol.

The volatilizable organic compounds are separated in the chromatograph by traversing the chromatographic column then are identified by the MS detector (mass spectrometry).

When a molecule is introduced into the mass spectrometer, its ionization produces several types of ions. We then obtain a mass spectrum which consists of the abundances of these ions (on the ordinate), as a function of their mass/charge ratio (on the abscissa).
The area of the chromatographic peaks of each compound is proportional to their content in the sample.

Benefits

Fast & simple,
Allows for precise identification (name, CAS number, probability of identification, etc.),
Allows access to the content (quantification, semi-quantification, relative area ratio),
Very sensitive (LQ from ppb to ppm),
Little sample required (a few µg or µL)