UPLC system : QSight LX50 (Perkin) MS-MS detector : QSight Triple Quadrupole (Perkin) Ionization modes : negative and positive ESI; APCI negative and positive Injector : 100 places thermostatically controlled autosampler Columns : UPLC Brownlee Hres C18 (100mm x 21mm; 1.9µm) & Hypercarb (100mm x 3mm; 5µm) & Quasar (100mm x 2.1mm; 2.6µm) Mass range : 50 - 1,500 m / z Mass accuracy : ≤ 0.1 g / mol Detection limit : About ppt
Principle
The injection in liquid chromatography will allow the molecules to be separated before their detection by the Mass spectrometer (MS). The area of the chromatographic peaks for each compound will be proportional to its content in the sample analyzed.
The Triple Quadrupole allows a quantitative analysis of all organic and semi-volatile compounds. It offers the possibility of fragmenting molecules and therefore different specific and complementary analytical modes:
Comprehensive ion scanning scans each m / z of a selected mass range
Scanning for ions after fragmentation allows us to identify an unknown substance in a complex mixture
The more sensitive and selective MRM (Multiple Reaction Monitoring) mode allows us to quantify many compounds simultaneously thanks to the monitoring of specific transitions.
Benefits
Save time thanks to simultaneous analysis of compounds
Selective and sensitive analysis with MRM mode
Disadvantages
No qualitative screening possible with a triple quadrupole, only analysis of "target" compounds.
Only molecules with heteroatoms are detectable (compounds of the family of simple aliphatics and aromatics are not detected)
Application example
identification and determination of biocides
Isothiazolinones, belonging to the biocides family, are frequently used in cosmetic products and detergents as preservatives and..
Identification and determination of biocides
identification and dosages of phthalates
Certain phthalates such as DBP, DIBP or even BBP have been classified as carcinogenic, mutagenic and reprotoxic substances by ECHA (European..
Identification and dosages of phthalates
determination of nitrosamines
Determination of nitrosamines by UPLC-MS / MS
Determination of nitrosamines
determination of acrylamide
The Polymex laboratory therefore suggests that you measure residual acrylamide by UPLC-MS / MS (Liquid Chromatography coupled with Tandem..
Determination of acrylamide
Our analytical resources
gcms-fid
During injection into the GC, all organic compounds will be separated by the chromatographic column and then identified by the MS (mass..
GCMS-FID
pyrolyzer
The sample is directly injected into the pyrolyzer oven heated to 550 ° C using a solid syringe. This instantly causes the fragmentation of..
PYROLYZER
uplc/uv/tof-ms
During the injection into the LC, all the semi-volatile organic molecules will be separated by the chromatographic column and then detected..
UPLC/UV/TOF-MS
uplc-ms-ms
During the injection into the LC module, all the semi-volatile organic molecules will be separated by the chromatographic column and then..
UPLC-MS-MS
headspace/gcms
The HeadSpace / GCMS technique does not require preparation. The sample is placed in a gas tight vial and hermetically sealed using a..
HEADSPACE/GCMS
irtf spectrometer
This spectral analysis makes it possible to know the nature of the covalent bonds present in the sample. Each of them (for example..
IRTF SPECTROMETER
sem/edx
A SEM (Scanning Electron Microscope) uses an electron beam to obtain enlarged images of very good resolution. It can also be used to..
SEM/EDX
centrifuge
CENTRIFUGE
uv-vis spectrometer
Certain molecules form by reaction colorimetric complexes having maximum absorption in UV-Visible. The color of these complexes is read..
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