UPLC/UV/TOF-MS

UPLC / UV / TOF-MS Altus Axion

UPLC system : Altus UPLC A-30 (Perkin)
TOF MS (Time Of Flight) detector : Axion TOF 2 (Perkin)
UV detector : Altus A30 (Perkin)
Ionization modes : negative and positive ESI; APCI negative and positive
Injector : 96-place thermostatically controlled autosampler
Column : UPLC Brownlee Hres C18 (100 mm x 21 mm x 1.9 µm) & Hypercarb 1 mm x 100 mm x 3µm
Pre-column : Altus UPLC BEH C18
Mass range : 18 - 16,000 m / z
Mass accuracy : ≤ 2 ppm for m / z 1000

Principle

During the injection into liquid chromatography, all the semi-volatile organic molecules will be separated by the chromatographic column and then detected by UV and TOF detectors (ESI or APCI mode). The area of the chromatographic peaks of each compound is proportional to their content in the sample.

The TOF module makes it possible to detect the molecular ion (in ionized form = adduct) of a molecule with an extremely precise molar mass (4-digit precision). Knowledge of this molecular mass gives access to proposals for a raw formula.

Benefits

Exact mass measurement (4-digit precision) and identification of unknown compounds,
Identification and quantification of organic compounds,
Very sensitive (Limit of quantification <5 ppb for Irganox 1010 in a PE)

Disadvantages

Only molecules with heteroatoms are detectable (compounds of the family of simple aliphatics and aromatics are not detected).

Application areas

Organic adjuvants of polymers such as Antioxidants, Anti-UV (Irganox 1010, Irganox 1076, Irgafos 168…),
Thermolabile or reactive compounds (Formaldehyde, Acrylamide, Diisocyanates, residual monomers, etc.),
Extractable and leachable according to ISO 10993 (medical devices, implantable…),
Deformulation of Surfactant and TensioActive
Determination of slip agents in plastic films (Fatty amides, Erucamide, Oleamide, Stereamide),
Determination of amines, diamines, amides (Hexamethylene diamine, Acrylamide, etc.) in polymers in solution.